In this research, in order to study the concentrations of heavy metals Cu, Zn, Pb, Ni, and Fe in Loach fish (Paracobitis rhadinaea), sampling was done in Chahnime water reservoirs. Afterwards, the fish were transported to the laboratories at Hamun International Wetland Research institute for preparation and digestion in order to measure the concentration of heavy metals. The concentration of heavy metals was measured using the Konic NOVAA 300 atomic absorption spectrophotometer on the basis of micrograms per gram dry weight. In the muscles, the concentrations of Cu, Fe, Ni, Pb, and Zn were measured to be 10.40±075, 196.04±10.88, 3.16±0.10, 4.35±0.68, and 118.04±50.08 µg per gram dry weight respectively. In the liver, the concentrations of Cu, Fe, Ni, Pb, and Zn were measured to be 35.27±16.13, 467.11±117.47, 15.15±3.69, 13.15±6.93, and 198.20±50.08 µg per gram dry weight respectively. Analysis of the acquired data showed that metal concentration pattern for liver and muscle tissues is similar and the highest concentrations were those of Fe and Zn. Concentrations of Cu, Pb, and Ni too did not show any significant difference between the two tissues. Pb, Fe, and Ni showed higher concentrations than the critical limits set by some standards (WHO and FDA) which could be stimulated by the environmental factors e.g. food, water and surrounding environment. The significant relationship between Zn concentration in liver and muscles shows its similar concentration pattern between both tissues and that changes which affect liver concentration would stimulate concentration changes in muscles too.